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規(guī)格

規(guī)格	2樣本、4樣本、8樣本、8樣本(產(chǎn)品加服務(wù))、4樣本(產(chǎn)品加服務(wù))、2樣本(產(chǎn)品加服務(wù))
樣本類型	Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
原理方法	Sandwich-based
檢測方法	Chemiluminescence
定量與否	Semi-Quantitative
載體	Membrane
適用種屬	Human
檢測靶標(biāo)	TNF alpha, IGF-1, IGFBP-2, IGF-1 R, IGFBP-1, IGFBP-3, bad, p53, bax, Bcl-2, Bcl-w, BID, BIM, BIRC4, Caspase-3, Caspase-8, cIAP-2, Cytochrome C, HSP27, HSP60, HSP70, HTRA2, IAP-3, IGF-2, IGFBP-4, IGFBP-5, IGFBP-6, Livin, Omi, p21, SMAC, XIAP, Fas Ligand / TNFSF6, TNF R1 / TNFRSF1A, CD40 Ligand / TNFSF5, TNF R2 / TNFRSF1B, CD40 / TNFRSF5, Fas / TNFRSF6 / Apo-1, DR6 / TNFRSF21, p27/Kip1 / CDKN1B, TNF beta / TNFSF1 / LT-alpha, TRAIL R1 / TNFRSF10A / DR4, TRAIL R2 / TNFRSF10B / DR5, TRAIL R3 / TNFRSF10C, TRAIL R4 / TNFRSF10D, Survivin / BIRC5
靶標(biāo)數(shù)	43

詳細描述

Product Features

Easy to use
No specialized equipment needed
Compatible with nearly any liquid sample
Proven technology (many publications)
Highly sensitive (pg/ml)
Sandwich ELISA specificity
Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
bad Gene Symbol: bad Gene ID: 572 Accession #: Q92934 ELISA Kit: RayBio Human BAD ELISA	bax Gene Symbol: BAX Gene ID: 581 Accession #: Q07812	bcl-2 Gene Symbol: BCL2 Gene ID: 596 Accession #: P10415	bcl-w Gene Symbol: BCL2L2 Gene ID: 599 Accession #: Q92843	BID Gene Symbol: BID Gene ID: 637 Accession #: P55957 ELISA Kit: RayBio Human BID ELISA
BIM Gene Symbol: BCL2L11 Gene ID: 10018 Accession #: Q43521	Caspase-3 Gene Symbol: CASP3 Gene ID: 836 Accession #: P42574	Caspase-8 Gene Symbol: CASP8 Gene ID: 841 Accession #: Q14790	CD40 (TNFRSF5) Gene Symbol: CD40 Gene ID: 958 Accession #: P25942 ELISA Kit: RayBio Human CD40 ELISA	CD40 Ligand (TNFSF5) Gene Symbol: CD40LG Gene ID: 959 Accession #: P29965 ELISA Kit: RayBio Human CD40L ELISA
cIAP-2 Gene Symbol: BIRC3 Gene ID: 330 Accession #: Q13489 ELISA Kit: RayBio Human cIAP2 ELISA	Cytochrome C Gene Symbol: CYCS Gene ID: 54205 Accession #: P99999 ELISA Kit: RayBio Custom Human Cytochrome C ELISA	DR6 (TNFRSF21) Gene Symbol: TNFRSF21 Gene ID: 27242 Accession #: O75509 ELISA Kit: RayBio Human DR6 ELISA	Fas (TNFRSF6/Apo-1) Gene Symbol: Fas Gene ID: 355 Accession #: P25445 ELISA Kit: RayBio Human Fas ELISA	Fas Ligand (TNFSF6) Gene Symbol: FASLG Gene ID: 356 Accession #: P48023 ELISA Kit: RayBio Human FasL ELISA
HSP27 Gene Symbol: HSPB1 Gene ID: 3315 Accession #: P04792 ELISA Kit: RayBio Human HSP27 ELISA	HSP60 Gene Symbol: HSPA14 Gene ID: 51182 Accession #: Q0VDF9	HSP70 Gene Symbol: HSPA4 Gene ID: 3308 Accession #: P34932 ELISA Kit: RayBio Human HSP70 ELISA	HTRA2 Gene Symbol: HTRA2 Gene ID: 27429 Accession #: O43464 ELISA Kit: RayBio Human HTRA2?ELISA	IGF-1 Gene Symbol: IGF1 Gene ID: 3479 Accession #: P05019 ELISA Kit: RayBio Human IGF-1 ELISA
IGF-2 Gene Symbol: Igf2 Gene ID: 3481 Accession #: P01344	IGFBP-1 Gene Symbol: IGFBP1 Gene ID: 3484 Accession #: P08833 ELISA Kit: RayBio Human IGFBP-1 ELISA	IGFBP-2 Gene Symbol: IGFBP2 Gene ID: 3485 Accession #: P18065 ELISA Kit: RayBio Human IGFBP-2 ELISA	IGFBP-3 Gene Symbol: IGFBP3 Gene ID: 3486 Accession #: P17936 ELISA Kit: RayBio Human IGFBP-3 ELISA	IGFBP-4 Gene Symbol: IGFBP4 Gene ID: 3487 Accession #: P22692 ELISA Kit: RayBio Human IGFBP-4 ELISA
IGFBP-5 Gene Symbol: IGFBP5 Gene ID: 3488 Accession #: P24593 ELISA Kit: RayBio Human IGFBP-5 ELISA	IGFBP-6 Gene Symbol: IGFBP6 Gene ID: 3489 Accession #: P24592 ELISA Kit: RayBio Human IGFBP-6 ELISA	IGF-1 R Gene Symbol: IGF1R Gene ID: 3480 Accession #: P08069 ELISA Kit: RayBio Human IGF-1 R ELISA	livin Gene Symbol: BIRC7 Gene ID: 79444 Accession #: Q96CA5 ELISA Kit: RayBio Human Livin ELISA	p21 Gene Symbol: CDKN1A Gene ID: 1026 Accession #: P38936
p27 Gene Symbol: SSSCA1 Gene ID: 10534 Accession #: O60232 ELISA Kit: RayBio Human p27 ELISA	p53 Gene Symbol: TP53 Gene ID: 7157 Accession #: P04637 ELISA Kit: RayBio Human p53 ELISA	SMAC Gene Symbol: DIABLO Gene ID: 56616 Accession #: Q9NR28 ELISA Kit: RayBio Human SMAC ELISA	Survivin (BIRC5) Gene Symbol: BIRC5 Gene ID: 332 Accession #: O15392	TNF RI (TNFRSF1A) Gene Symbol: TNFRSF1A Gene ID: 7132 Accession #: P19438 ELISA Kit: RayBio Human sTNFRI ELISA
TNF RII (TNFRSF1B) Gene Symbol: TNFRSF1B Gene ID: 7133 Accession #: P20333 ELISA Kit: RayBio Human sTNFRII ELISA	TNF alpha Gene Symbol: TNF Gene ID: 7124 Accession #: P01375 ELISA Kit: RayBio Human TNF-alpha ELISA	TNF beta (TNFSF1B) Gene Symbol: LTA Gene ID: 4049 Accession #: P01374 ELISA Kit: RayBio Human TNF-beta ELISA	TRAIL R1 (TNFRSF10A/DR4) Gene Symbol: TNFRSF10A Gene ID: 8797 Accession #: O00220 ELISA Kit: RayBio Human TRAIL R1?ELISA	TRAIL R2 (TNFRSF10B/DR5) Gene Symbol: TNFRSF10B Gene ID: 8795 Accession #: O14763 ELISA Kit: RayBio Human TRAIL R2?ELISA
TRAIL R3 (TNFRSF10C) Gene Symbol: TNFRSF10C Gene ID: 8794 Accession #: O14798 ELISA Kit: RayBio Human TRAIL R3 ELISA	TRAIL R4 (TNFRSF10D) Gene Symbol: TNFRSF10D Gene ID: 8793 Accession #: Q9UBN6 ELISA Kit: RayBio Human TRAIL R4 ELISA	XIAP Gene Symbol: XIAP Gene ID: 331 Accession #: P98170 ELISA Kit: RayBio Human XIAP ELISA

Application Notes

Suggested Application

Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process

Kit Components

Human Apoptosis Antibody Array C1 Membranes
Blocking Buffer
Wash Buffer 1
Wash Buffer 2
Biotinylated Detection Antibody Cocktail
Streptavidin-Conjugated HRP
Detection Buffer C
Detection Buffer D
Lysis Buffer
Protease Inhibitor Cocktail
8-Well Incubation Tray
Plastic Sheets
Array Templates
Manual

Other Materials Required

Pipettors, pipet tips and other common lab consumables
Orbital shaker or oscillating rocker
Tissue Paper, blotting paper or chromatography paper
Adhesive tape or Saran Wrap
Distilled or de-ionized water
A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It^? or EpiChem II Benchtop Darkroom or GE's ImageQuant^? LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.

Protocol Outline

Block membranes
Incubate with Sample
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with HRP-Conjugated Streptavidin
Incubate with Detection Buffers
Image with chemiluminescent imaging system
Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

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文件

AAH-APO-1.pdf

文獻(12)

Citicoline Eye Drops Protect Trabecular Meshwork Cells from Oxidative Stress Injury in a 3D In Vitro Glaucoma Model,Marote Ana, Santos Diogo, Mendes-Pinheiro Bárbara, Serre-Miranda Cláudia, Anjo Sandra I., Vieira Joana, Ferreira-Antunes Filipa, Correia Joana Sofia, Borges-Pereira Caroline, Pinho Andreia G., Campos Jonas, Manadas Bruno, Teixeira Manuel R., Correia-Neves Margarida, Pinto Luísa, Costa Pedro M., Roybon Laurent, Salgado António J.,Stem Cell Reviews and Reports ,(IF:6.692)
Species：	Human
Sample Type：	unknown

View publications

Evaluation of cytotoxic and chemotherapeutic properties of boldine in breast cancer using in vitro and in vivo models,Deborah F. Kelly, G M Jonaid, Liam Kaylor, Maria J. Solares, Samantha Berry, Liza-Anastasia DiCecco, William Dearnaley, Michael Casasanta,Micromachines ,(IF:3.4)
Species：	Human
Sample Type：	unknown

View publications

In vitro and in vivo anti-angiogenic activity of girinimbine isolated from Murraya koenigii,Jingyu Ren, Tao Liu, Bo Bi, Saba Sohail, Fakhar ud Din,JOURNAL OF PHARMACEUTICAL SCIENCES ,(IF:3.8) 作者單位：陽煤集團總醫(yī)院
Species：	Human
Sample Type：	unknown

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Knockdown of CHPF suppresses cell progression of non-small-cell lung cancer,Jennifer L. Yates, Danielle T. Hunt, Karen E. Kulas, Karen J. Chave, Linda Styer, Sandhya T. Chakravarthi, Gianna Y. Cai, Maria C. Bermúdez-González, Giulio Kleiner, Deena Altman, Komal Srivastava, , Viviana Simon, Dennis Feihel, Joseph McGowan, Wayne Hogrefe, Philip Noone, Christina Egan, Mark K. Slifka, William T. Lee,JOURNAL OF MEDICAL VIROLOGY ,(IF:12.7)
Species：	Human
Sample Type：	unknown

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Koenimbin, a natural dietary compound of Murraya koenigii (L) Spreng: inhibition of MCF7 breast cancer cells and targeting of derived MCF7 breast cancer stem cells (CD44+/CD24?/low): an in vitro study,Afonso Malheiro, Adrián Seijas-Gamardo, Abhishek Harichandan, Carlos Mota, Paul Wieringa, Lorenzo Moroni,ACS Applied Materials & Interfaces ,(IF:10.383)
Species：	Human
Sample Type：	unknown

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α-Mangostin from Cratoxylum arborescens demonstrates apoptogenesis in MCF-7 with regulation of NF-κB and Hsp70 protein modulation in vitro, and tumor reduction in vivo,Goekhan Yuecel, Xiaobo Zhou, Linda Terkatz, Angela Wendel, Julius Reinhardt, Ibrahim El-Battrawy, Katherine Sattler, Lukas Cyganek, Jochen Utikal, Harald Langer, Ruediger Scharf, Daniel Duerschmied, Ibrahim Akin,INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES ,(IF:5.6)
Species：	Human
Sample Type：	unknown

View publications

Linalool-Loaded Glutathione-Modified Gold Nanoparticles Conjugated with CALNN Peptide as Apoptosis Inducer and NF-κB Translocation Inhibitor in SKOV-3 Cell Line,Vivienne Theobald, Ekkehard Grünig, Nicola Benjamin, Hans-Jürgen Seyfarth, Michael Halank, Marc A. Schneider, Sarah Richtmann, Daniel Kazdal, Katrin Hinderhofer, Panagiota Xanthouli, Benjamin Egenlauf, Satenik Harutyunova, Marius M. Hoeper, Danny Jonigk, Richard Sparla, Martina U. Muckenthaler, Christina A. Eichstaedt,Pulmonary Circulation ,(IF:2.6)
Species：	Human
Sample Type：	unknown

View publications

Novel combination treatment of CDK 4/6 inhibitors with PARP inhibitors in triple negative breast cancer cells,Cláudia Raquel Marques, Miguel de Almeida Fuzeta, Raquel Medina dos Santos Cunha, Joana Pereira-Sousa, Deolinda Silva, Jonas Campos, Andreia Teixeira-Castro, Rui Amandi Sousa, Ana Fernandes-Platzgummer, Cláudia L. da Silva, António José Salgado,Biomedicines ,(IF:4.7)
Species：	Human
Sample Type：	unknown

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Polyphenolic Carbon Quantum Dots with Intrinsic Reactive Oxygen Species Amplification for Two-Photon Bioimaging and In Vivo Tumor Therapy,Takeshi Chida, Yuji Ishida, Sho Morioka, Go Sugahara, Christine Han, Bill Lam, Chihiro Yamasaki, Remi Sugahara, Meng Li, Yasuhito Tanaka, T. Jake Liang, Chise Tateno, Takeshi Saito,JCI Insight ,(IF:8)
Species：	Mouse
Sample Type：	unknown

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Specific Forms of Graphene Quantum Dots Induce Apoptosis and Cell Cycle Arrest in Breast Cancer Cells,Junchao Duan, Yongbo Yu, Yang Yu, Yang Li, Ji Wang, Weijia Geng, Lizhen Jiang, Qiuling Li, Xianqing Zhou, Zhiwei Sun,International Journal of Nanomedicine ,(IF:8) 作者單位：首都醫(yī)科大學(xué)
Species：	Human
Sample Type：	unknown

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Statins inhibit proliferation and induce apoptosis in triple-negative breast cancer cells,Cheng Zhou, Xinlong Bai, Yunzhao Yang, Meihan Shi, Xue-Yuan Bai,STEM CELLS AND DEVELOPMENT ,(IF:4) 作者單位：中國人民解放軍總醫(yī)院（301醫(yī)院）
Species：	Human
Sample Type：	unknown

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The apoptotic effects of Brucea javanica fruit extract against HT29 cells associated with p53 upregulation and inhibition of NF-κB translocation,Bijoy Krishna Das, Sourav Ghosh, Antony Gomes, Utpal Chandra De,Inorganic and Nano-Metal Chemistry ,(IF:1.7)
Species：	Human
Sample Type：	unknown

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Product Features

Target Names

Application Notes

Storage/Stability